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Data and Experimental Skills

Topic 8 of 8

Organic apparatus and tests

Connect reflux, distillation and observations to the chemistry.

A-Level 9476 (2026-2027)

Connect organic tests to apparatus and purification

Preserve volatile reactants, isolate a product and interpret functional-group evidence.

Reflux, then isolate
  1. Reflux for sustained heating

    A vertical water-cooled condenser returns condensed vapour to the reaction flask. Keep the apparatus open to the atmosphere through the condenser; never seal a heated system.

  2. Distil when collecting a volatile fraction

    Rearrange appropriately so vapour passes into a condenser and receiver. Put the thermometer bulb at the vapour entrance to the condenser when measuring the distillation temperature.

  3. Control heating and cooling

    Run condenser water in at the lower connection and out at the upper. Use the specified suitable heat source, such as a water bath for an appropriate volatile/flammable system, and add anti-bumping material before heating when required.

  4. Purify and assess

    Choose the supplied separation/purification method from the product properties. Yield measures recovered amount; an appropriate boiling/melting behaviour or other supplied evidence addresses purity.

Reflux returns condensed liquid to the reaction

A vertical condenser is open at the top. Vapour rises through its inner tube; cooled liquid runs back to the heated flask. Cooling water enters the lower jacket connection and leaves the upper connection. A water bath is shown as one suitable heat source.

Schematic: supports and clamps are omitted. Vapour and cooling water travel in separate spaces. Choose a water bath only when it can provide the required reaction temperature.

Distillation sends the condensate to a receiver

Vapour leaves a heated flask through a side arm, passes the thermometer bulb, then condenses in a downward-sloping condenser and enters a separate open receiver. Cooling water enters at the low end and leaves at the high end.

Schematic: supports and clamps are omitted. The thermometer measures the departing vapour. Keep the receiver open to the atmosphere and use the specified heat source; a water bath cannot provide temperatures above its boiling point.

Worked example

Choose a purification step from the mixture

A supplied synthesis gives a neutral organic liquid P that is almost insoluble in water and does not react with a dilute carbonate wash. It is mixed with residual acid, water and a more volatile organic solvent. The question supplies distinct boiling ranges for P and the solvent.

  1. Use a separating funnel to separate the aqueous and organic phases after the specified wash. The acid reacts with carbonate and enters the aqueous phase as dissolved ions; vent the funnel appropriately because carbon dioxide is formed. Identify which layer contains P from the supplied density or a water-drop test, rather than assuming the organic layer is always on top.
  2. Retain the P-containing layer. Remove residual dissolved water with a suitable anhydrous drying agent that does not react with P, then remove that solid. Drying is different from removing an entire aqueous layer.
  3. Distil the dried liquid using the specified appropriate heat source. Collect the solvent fraction separately, then collect P over its supplied boiling range. This choice depends on a sufficient volatility difference and thermal stability.
  4. If P were instead a solid soluble in hot solvent but sparingly soluble when cold, choose recrystallisation: dissolve in minimal hot solvent, remove insoluble impurities if needed, cool to form crystals, then filter, wash sparingly with cold solvent and dry.
Answer

Each operation removes a named impurity by a stated property. Reflux helps a reaction proceed; it does not itself isolate a pure product.

Organic tests: observation before deduction
Test contextPositive evidenceInterpretation
Bromine water with an alkeneOrange solution is decolourisedConsistent with C=C, but other reactive groups may also consume bromine
Halogenoalkane hydrolysis then halide testHeat with aqueous NaOH; acidify with dilute HNO3, then add AgNO3Precipitate colour supports the released halide; the covalently bound halogen is not tested directly as a free ion
Primary or secondary alcohol with heated acidified manganate(VII)Purple colour is removedOxidation occurs; tertiary alcohols resist this oxidation under the usual conditions
Carbonyl compound with 2,4-DNPHOrange precipitateCarbonyl evidence; reagent excluded from hands-on H2 assessment
Aldehyde with Tollens reagent on warmingSilver mirrorAldehyde oxidation; follow the supplied handling/disposal instructions
Aliphatic aldehyde with Fehling solution on warmingRed-brown precipitateDistinguishes it from a typical ketone under these conditions
Methyl ketone or appropriate alcohol with alkaline iodine, warmPale-yellow precipitateIodoform evidence for CH3CO- or CH3CH(OH)-; ethanol/ethanal also give the test
Carboxylic acid with aqueous carbonateCO2 evolves and is confirmed with limewaterAcidic enough to react with carbonate
Primary amide heated with aqueous NaOHAmmonia evolvesHydrolysis releases NH3; test with damp red litmus
Phenol or phenylamine with bromine waterDecolourisation and a white precipitateContext is needed to distinguish the two; practical tests involving phenol itself are excluded

Solid PCl5 gives steamy HCl fumes with suitable hydroxy compounds, including alcohols and carboxylic acids; it is not a unique alcohol test. PCl5, 2,4-DNPH and phenol are excluded from hands-on H2 tests. Large-scale synthesis involving reflux, distillation and purification is also not expected as an examination operation, although the principles and apparatus remain relevant.

Check your understandingWhy would immediate distillation be unsuitable when a volatile reactant needs prolonged heating with another reactant?Think it through, then reveal the answer
It would remove the volatile material from the reacting mixture. Reflux returns condensed vapour so heating can continue; distillation is useful later when a volatile fraction is to be collected.