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Respiration and ATP production

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What is the net glycolytic ATP yield per glucose?

  1. Zero because all ATP comes from mitochondria
  2. Two
  3. Four, with no ATP investment
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Two

Four produced minus two invested gives two net.

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Where is proton concentration elevated by the respiratory chain?

  1. The intermembrane space relative to the matrix
  2. The cytosol across the outer mitochondrial membrane
  3. The matrix because all protons are pumped inward
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The intermembrane space relative to the matrix

The chain pumps protons out of the matrix across the inner membrane.

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Which pair is correct?

  1. Both pathways require oxygen as their final acceptor.
  2. Yeast ethanol formation releases CO2; lactate formation does not.
  3. Muscle lactate formation releases CO2; yeast fermentation never does.
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Yeast ethanol formation releases CO2; lactate formation does not.

Yeast decarboxylates pyruvate to ethanal before reducing it.

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Which statement about fermentation in yeast is NOT correct?

  1. It produces ethanol
  2. It produces extra ATP from pyruvate
  3. It releases carbon dioxide
  4. It regenerates NAD for glycolysis
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It produces extra ATP from pyruvate

The steps after pyruvate make no ATP. Only the two net ATP from glycolysis are made.

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Why include a matched non-respiring control?

  1. To absorb the CO2 instead of using a suitable absorbent
  2. To estimate non-biological effects on gas movement
  3. To replace repeated measurements of the living sample
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To estimate non-biological effects on gas movement

This helps correct for temperature or pressure changes.

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Question 1 of 8

Account per glucose

3 marks

State CO2 output from glycolysis, link reactions and Krebs cycle per glucose, and the total reduced NAD produced across these stages before oxidative phosphorylation. [3]

Use two pyruvate and two Krebs turns per glucose.

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