Chapter 9 / 3 short lessons
DNA amplification and analysis
Choose PCR, a gel or a probe for the question, and interpret their controls honestly.
H2 Biology / 9477 / 2027 syllabus
What do you think?
A band has the expected size. Does that prove its exact DNA sequence?
Lessons
0 of 3 marked as readPCR amplifies a chosen DNA region
Explain PCR ingredients, thermal cycles, advantages and limitations.
About 8 minSeparate DNA by fragment length
Explain agarose electrophoresis and interpret bands with controls.
About 7 minFind a sequence with a probe
Explain Southern blotting and nucleic-acid hybridisation.
About 7 minWhat you will be able to do
- Explain the stages and limits of PCR.
- Read gel direction, sizes and controls.
- Use complementary probes to identify target DNA.
Spaced review
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