9477 / 2027

Lesson 2 of 5 / Population genetics

Build the Hardy-Weinberg model

Why is the heterozygote frequency 2pq?

In this lesson: Connect random gamete combinations to p squared, 2pq and q squared.

About 6 min

The key ideaRandom combination of gametes gives AA = p squared, Aa = 2pq and aa = q squared, when the model applies.

Explore the idea

Two routes make a heterozygote

A: 0.60a: 0.40A: 0.60a: 0.40AA36.0%Aa24.0%Aa24.0%aa16.0%Add the two shaded Aa cells

Aa = pq + qp = 48.0%. Moving the slider changes allele frequencies; it keeps the random-mating model in place.

Explanation

The Hardy-Weinberg model is a reference for inheritance at a locus in a population. With two alleles, let their frequencies be p and q, so p + q = 1. Under the model, allele frequencies remain stable between generations and genotype frequencies follow a predictable distribution.

Imagine drawing an allele independently from each of two gamete pools with the same allele frequencies. The probability of drawing A from both is p x p, giving p squared for AA. Drawing a from both gives q squared for aa.

A heterozygote can form in two ways: A from the first gamete and a from the second, or a from the first and A from the second. These mutually exclusive possibilities each have probability pq, so their total is 2pq. They are two routes to one genotype, not two different genotypes.

The three proportions sum to 1 because they include every possible genotype: p squared + 2pq + q squared = 1. Multiply a proportion by the population size to obtain an expected number of individuals. Expected numbers describe the model; actual samples can vary by chance.

If A is completely dominant, the A phenotype includes AA and Aa. Its expected frequency is p squared + 2pq, also equal to 1 - q squared. With codominance, the heterozygote has a distinguishable phenotype, so there are three phenotype classes. Match the genotype-to-phenotype rule given in the question.

Step by step
  1. 1

    Start with allele frequencies

    Define p and q before substituting numbers.

  2. 2

    Combine independent gametes

    Multiply frequencies for each combination and add the two heterozygote routes.

  3. 3

    Translate to the requested quantity

    Keep genotype proportions, phenotype proportions and expected counts distinct.

Worked example

Work through the evidence

For p = 0.6 and q = 0.4, predict genotype numbers in 500 individuals under Hardy-Weinberg equilibrium.

One way to explain it

AA: 0.6 squared x 500 = 180. Aa: 2 x 0.6 x 0.4 x 500 = 240. aa: 0.4 squared x 500 = 80. The expected counts sum to 500.

Why this answer works
  • Square each allele frequency only for its homozygote.
  • Include both routes to the heterozygote.
  • Convert proportions to counts only at the last step.
Is this true? "2pq represents two different kinds of heterozygote."

Aa and aA are the same genotype in this autosomal model. The two products count the two possible parental origins of the alleles.

Try a question

When p = q = 0.5, which expected genotype distribution follows?
You can return to this lesson any time.